FEATURES

FAMILY

DESCRIPTION

Also known as TSAE_BACSU, tsaE, ydiB. Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB; this reaction does not require ATP in vitro. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro, which is modulated by the oligomeric status of the protein. Monomer, homodimer or homotetramer; in equilibrium. Low salt concentration favors oligomerization, while high salt concentration favors the monomeric form.

EXTERNAL LINKS

tsaE

tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

tsaE

Molecular Synopsis